One goal of our lab is to share simple, inexpensive, and hopefully powerful tools for biological research. Here are a few:

Knockout Sudoku

Buz Barstow and Michael developed Knockout Sudoku, a method for the construction of whole-genome knockout collections for a wide range of microorganisms with as little as three weeks of dedicated labor and at a cost of approximately $10,000.

Buz's lab made a step-by-step protocol for Knockout Sudoku


Inexpensive Genomic Library Preparation for Microbes

Michael, along with Tami Lieberman and Hattie Chung in the Kishony lab, and Sergei Kryazhimskiy in Michael Desai's lab developed a protocol for running Nextera XP genomic library preparation at vastly smaller volumes. When run in high-throughput this allows preparation of genomic DNA for sequencing for under $10 a sample.

Step-by-step protocol for inexpensive library prep


The “Macroscope” Imaging Platform

Remy Chait and Michael in the Kishony lab built a high-throughput fluorescent imaging platform using a consumer camera, LEDs, and filters. The system can be augmented with a robotic arm for high-throughput use.

Macroscope parts list and photos (Open Wetware)


The MEGA-plate

Michael, Tami Lieberman, and many others in the Kishony lab built the MEGA-plate, a giant petri dish for visualizing and studying microbial evolution.

MEGA-plate building information (Open Wetware)